t bruker biospec 94 20 Search Results


dounce homogenizer  (Cole-Parmer)
96
Cole-Parmer dounce homogenizer
Dounce Homogenizer, supplied by Cole-Parmer, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/t+bruker+biospec+94+20/1200C+GenoLyte+Temperature+Controlled+Compact+Homogenizer/10__1074_slash_jbc__m304913200-68-38-52
Average 96 stars, based on 1 article reviews
dounce homogenizer - by Bioz Stars, 2026-09
96/100 stars
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batimastat cas 130370-60-4  (Merck KGaA)
90
Merck KGaA batimastat cas 130370-60-4
MMP and ADAM10 inhibitors do not block HpHtrA activity. A NCI-N87 cells were infected with H. pylori strains P12 wt, N6 wt and N6 ΔhtrA for 24 h. Where indicated, cells were treated with the broad range MMP inhibitor <t>Batimastat</t> (Bat) and ADAM10 inhibitor GI254023X (GI). Supernatants of infected cells were analyzed by gelatin zymography (upper panel) for proteolytic activity of activated MMPs and by casein zymography (lower panel) for proteolytic activity of secreted HpHtrA from H. pylori P12 wt and N6 wt. As a control, 10 µg of bacterial lysates of H. pylori P12 wt, N6 wt and N6 ΔhtrA were loaded on the zymograms. B NCI-N87 cells were stimulated for 24 h with 50 ng/ml TNFα to induce MMP activation. Where indicated, cells were additionally treated with broad range MMP inhibitor Batimastat (Bat), ADAM10/17 inhibitor GI254023X (GI) or a combination of both (Bat/GI). As a control, cells were incubated with 0.1% of the solvent DMSO. Supernatants of NCI-N87 cells were analyzed by Western blot for N-terminal fragments of hDsg2 (Dsg2 NTF ) and hCdh1 (Cdh1 NTF ) using antibodies recognizing the extracellular domains of hDsg2 and hCdh1. The overlay of hDsg2 (green) and hCdh1 (red) is presented to exclude possible antibody cross reactions. C 50 ng rCdh1 were incubated with 100 ng recombinant human MMP-7 for 16 h at 37 °C. As indicated, increasing concentrations of the broad range MMP inhibitor Batimastat (Bat) were added. Proteins were separated by SDS-PAGE and analyzed by Western blot. Full length rCdh1 (rCdh1 FL ) and cleavage fragments were detected using an antibody recognizing the extracellular domain of hCdh1. D 50 ng rCdh1 was incubated with 250 ng HpHtrA wt, inactive mutant (SA) or 100 ng active MMP-7 for 16 h at 37 °C. Where indicated broad range MMP inhibitor Batimastat (Bat), ADAM10 inhibitor GI254023X (GI) or a combination of both (Bat/GI) was added. Proteins were separated by SDS-PAGE and analyzed by Western blot. Full length rCdh1 (rCdh1 FL ) and cleavage fragments were detected using an antibody recognizing the extracellular domain of hCdh1. HpHtrA was detected with a polyclonal HpHtrA antibody
Batimastat Cas 130370 60 4, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/t+bruker+biospec+94+20/batimastat+cas+130370+60+4/pmc08571890-182-34-63
Average 90 stars, based on 1 article reviews
batimastat cas 130370-60-4 - by Bioz Stars, 2026-09
90/100 stars
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ictalurus punctatus alabama c91 20 ictalurus punctatus alabama  (ATCC)
94
ATCC ictalurus punctatus alabama c91 20 ictalurus punctatus alabama
MMP and ADAM10 inhibitors do not block HpHtrA activity. A NCI-N87 cells were infected with H. pylori strains P12 wt, N6 wt and N6 ΔhtrA for 24 h. Where indicated, cells were treated with the broad range MMP inhibitor <t>Batimastat</t> (Bat) and ADAM10 inhibitor GI254023X (GI). Supernatants of infected cells were analyzed by gelatin zymography (upper panel) for proteolytic activity of activated MMPs and by casein zymography (lower panel) for proteolytic activity of secreted HpHtrA from H. pylori P12 wt and N6 wt. As a control, 10 µg of bacterial lysates of H. pylori P12 wt, N6 wt and N6 ΔhtrA were loaded on the zymograms. B NCI-N87 cells were stimulated for 24 h with 50 ng/ml TNFα to induce MMP activation. Where indicated, cells were additionally treated with broad range MMP inhibitor Batimastat (Bat), ADAM10/17 inhibitor GI254023X (GI) or a combination of both (Bat/GI). As a control, cells were incubated with 0.1% of the solvent DMSO. Supernatants of NCI-N87 cells were analyzed by Western blot for N-terminal fragments of hDsg2 (Dsg2 NTF ) and hCdh1 (Cdh1 NTF ) using antibodies recognizing the extracellular domains of hDsg2 and hCdh1. The overlay of hDsg2 (green) and hCdh1 (red) is presented to exclude possible antibody cross reactions. C 50 ng rCdh1 were incubated with 100 ng recombinant human MMP-7 for 16 h at 37 °C. As indicated, increasing concentrations of the broad range MMP inhibitor Batimastat (Bat) were added. Proteins were separated by SDS-PAGE and analyzed by Western blot. Full length rCdh1 (rCdh1 FL ) and cleavage fragments were detected using an antibody recognizing the extracellular domain of hCdh1. D 50 ng rCdh1 was incubated with 250 ng HpHtrA wt, inactive mutant (SA) or 100 ng active MMP-7 for 16 h at 37 °C. Where indicated broad range MMP inhibitor Batimastat (Bat), ADAM10 inhibitor GI254023X (GI) or a combination of both (Bat/GI) was added. Proteins were separated by SDS-PAGE and analyzed by Western blot. Full length rCdh1 (rCdh1 FL ) and cleavage fragments were detected using an antibody recognizing the extracellular domain of hCdh1. HpHtrA was detected with a polyclonal HpHtrA antibody
Ictalurus Punctatus Alabama C91 20 Ictalurus Punctatus Alabama, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/t+bruker+biospec+94+20/Vibrio+marinovulgaris+ZoBell+and+Upham/pmc07377616-148-94-88
Average 94 stars, based on 1 article reviews
ictalurus punctatus alabama c91 20 ictalurus punctatus alabama - by Bioz Stars, 2026-09
94/100 stars
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wakogel c-500hg  (FUJIFILM)
90
FUJIFILM wakogel c-500hg
MMP and ADAM10 inhibitors do not block HpHtrA activity. A NCI-N87 cells were infected with H. pylori strains P12 wt, N6 wt and N6 ΔhtrA for 24 h. Where indicated, cells were treated with the broad range MMP inhibitor <t>Batimastat</t> (Bat) and ADAM10 inhibitor GI254023X (GI). Supernatants of infected cells were analyzed by gelatin zymography (upper panel) for proteolytic activity of activated MMPs and by casein zymography (lower panel) for proteolytic activity of secreted HpHtrA from H. pylori P12 wt and N6 wt. As a control, 10 µg of bacterial lysates of H. pylori P12 wt, N6 wt and N6 ΔhtrA were loaded on the zymograms. B NCI-N87 cells were stimulated for 24 h with 50 ng/ml TNFα to induce MMP activation. Where indicated, cells were additionally treated with broad range MMP inhibitor Batimastat (Bat), ADAM10/17 inhibitor GI254023X (GI) or a combination of both (Bat/GI). As a control, cells were incubated with 0.1% of the solvent DMSO. Supernatants of NCI-N87 cells were analyzed by Western blot for N-terminal fragments of hDsg2 (Dsg2 NTF ) and hCdh1 (Cdh1 NTF ) using antibodies recognizing the extracellular domains of hDsg2 and hCdh1. The overlay of hDsg2 (green) and hCdh1 (red) is presented to exclude possible antibody cross reactions. C 50 ng rCdh1 were incubated with 100 ng recombinant human MMP-7 for 16 h at 37 °C. As indicated, increasing concentrations of the broad range MMP inhibitor Batimastat (Bat) were added. Proteins were separated by SDS-PAGE and analyzed by Western blot. Full length rCdh1 (rCdh1 FL ) and cleavage fragments were detected using an antibody recognizing the extracellular domain of hCdh1. D 50 ng rCdh1 was incubated with 250 ng HpHtrA wt, inactive mutant (SA) or 100 ng active MMP-7 for 16 h at 37 °C. Where indicated broad range MMP inhibitor Batimastat (Bat), ADAM10 inhibitor GI254023X (GI) or a combination of both (Bat/GI) was added. Proteins were separated by SDS-PAGE and analyzed by Western blot. Full length rCdh1 (rCdh1 FL ) and cleavage fragments were detected using an antibody recognizing the extracellular domain of hCdh1. HpHtrA was detected with a polyclonal HpHtrA antibody
Wakogel C 500hg, supplied by FUJIFILM, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/t+bruker+biospec+94+20/wakogel+c+500hg/pm39619561-59-1-21
Average 90 stars, based on 1 article reviews
wakogel c-500hg - by Bioz Stars, 2026-09
90/100 stars
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primer extension  (Thermo Fisher)
99
Thermo Fisher primer extension
MMP and ADAM10 inhibitors do not block HpHtrA activity. A NCI-N87 cells were infected with H. pylori strains P12 wt, N6 wt and N6 ΔhtrA for 24 h. Where indicated, cells were treated with the broad range MMP inhibitor <t>Batimastat</t> (Bat) and ADAM10 inhibitor GI254023X (GI). Supernatants of infected cells were analyzed by gelatin zymography (upper panel) for proteolytic activity of activated MMPs and by casein zymography (lower panel) for proteolytic activity of secreted HpHtrA from H. pylori P12 wt and N6 wt. As a control, 10 µg of bacterial lysates of H. pylori P12 wt, N6 wt and N6 ΔhtrA were loaded on the zymograms. B NCI-N87 cells were stimulated for 24 h with 50 ng/ml TNFα to induce MMP activation. Where indicated, cells were additionally treated with broad range MMP inhibitor Batimastat (Bat), ADAM10/17 inhibitor GI254023X (GI) or a combination of both (Bat/GI). As a control, cells were incubated with 0.1% of the solvent DMSO. Supernatants of NCI-N87 cells were analyzed by Western blot for N-terminal fragments of hDsg2 (Dsg2 NTF ) and hCdh1 (Cdh1 NTF ) using antibodies recognizing the extracellular domains of hDsg2 and hCdh1. The overlay of hDsg2 (green) and hCdh1 (red) is presented to exclude possible antibody cross reactions. C 50 ng rCdh1 were incubated with 100 ng recombinant human MMP-7 for 16 h at 37 °C. As indicated, increasing concentrations of the broad range MMP inhibitor Batimastat (Bat) were added. Proteins were separated by SDS-PAGE and analyzed by Western blot. Full length rCdh1 (rCdh1 FL ) and cleavage fragments were detected using an antibody recognizing the extracellular domain of hCdh1. D 50 ng rCdh1 was incubated with 250 ng HpHtrA wt, inactive mutant (SA) or 100 ng active MMP-7 for 16 h at 37 °C. Where indicated broad range MMP inhibitor Batimastat (Bat), ADAM10 inhibitor GI254023X (GI) or a combination of both (Bat/GI) was added. Proteins were separated by SDS-PAGE and analyzed by Western blot. Full length rCdh1 (rCdh1 FL ) and cleavage fragments were detected using an antibody recognizing the extracellular domain of hCdh1. HpHtrA was detected with a polyclonal HpHtrA antibody
Primer Extension, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/t+bruker+biospec+94+20/Agarose/pmc08560871-63-33-64
Average 99 stars, based on 1 article reviews
primer extension - by Bioz Stars, 2026-09
99/100 stars
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ptc-200 peltier thermal cycler  (MJ Research)
90
MJ Research ptc-200 peltier thermal cycler
MMP and ADAM10 inhibitors do not block HpHtrA activity. A NCI-N87 cells were infected with H. pylori strains P12 wt, N6 wt and N6 ΔhtrA for 24 h. Where indicated, cells were treated with the broad range MMP inhibitor <t>Batimastat</t> (Bat) and ADAM10 inhibitor GI254023X (GI). Supernatants of infected cells were analyzed by gelatin zymography (upper panel) for proteolytic activity of activated MMPs and by casein zymography (lower panel) for proteolytic activity of secreted HpHtrA from H. pylori P12 wt and N6 wt. As a control, 10 µg of bacterial lysates of H. pylori P12 wt, N6 wt and N6 ΔhtrA were loaded on the zymograms. B NCI-N87 cells were stimulated for 24 h with 50 ng/ml TNFα to induce MMP activation. Where indicated, cells were additionally treated with broad range MMP inhibitor Batimastat (Bat), ADAM10/17 inhibitor GI254023X (GI) or a combination of both (Bat/GI). As a control, cells were incubated with 0.1% of the solvent DMSO. Supernatants of NCI-N87 cells were analyzed by Western blot for N-terminal fragments of hDsg2 (Dsg2 NTF ) and hCdh1 (Cdh1 NTF ) using antibodies recognizing the extracellular domains of hDsg2 and hCdh1. The overlay of hDsg2 (green) and hCdh1 (red) is presented to exclude possible antibody cross reactions. C 50 ng rCdh1 were incubated with 100 ng recombinant human MMP-7 for 16 h at 37 °C. As indicated, increasing concentrations of the broad range MMP inhibitor Batimastat (Bat) were added. Proteins were separated by SDS-PAGE and analyzed by Western blot. Full length rCdh1 (rCdh1 FL ) and cleavage fragments were detected using an antibody recognizing the extracellular domain of hCdh1. D 50 ng rCdh1 was incubated with 250 ng HpHtrA wt, inactive mutant (SA) or 100 ng active MMP-7 for 16 h at 37 °C. Where indicated broad range MMP inhibitor Batimastat (Bat), ADAM10 inhibitor GI254023X (GI) or a combination of both (Bat/GI) was added. Proteins were separated by SDS-PAGE and analyzed by Western blot. Full length rCdh1 (rCdh1 FL ) and cleavage fragments were detected using an antibody recognizing the extracellular domain of hCdh1. HpHtrA was detected with a polyclonal HpHtrA antibody
Ptc 200 Peltier Thermal Cycler, supplied by MJ Research, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/t+bruker+biospec+94+20/ptc+100+thermocycler/10__1074_slash_jbc__m800299200-58-90-94
Average 90 stars, based on 1 article reviews
ptc-200 peltier thermal cycler - by Bioz Stars, 2026-09
90/100 stars
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chlorpropamide  (Enamine Ltd)
94
Enamine Ltd chlorpropamide
MMP and ADAM10 inhibitors do not block HpHtrA activity. A NCI-N87 cells were infected with H. pylori strains P12 wt, N6 wt and N6 ΔhtrA for 24 h. Where indicated, cells were treated with the broad range MMP inhibitor <t>Batimastat</t> (Bat) and ADAM10 inhibitor GI254023X (GI). Supernatants of infected cells were analyzed by gelatin zymography (upper panel) for proteolytic activity of activated MMPs and by casein zymography (lower panel) for proteolytic activity of secreted HpHtrA from H. pylori P12 wt and N6 wt. As a control, 10 µg of bacterial lysates of H. pylori P12 wt, N6 wt and N6 ΔhtrA were loaded on the zymograms. B NCI-N87 cells were stimulated for 24 h with 50 ng/ml TNFα to induce MMP activation. Where indicated, cells were additionally treated with broad range MMP inhibitor Batimastat (Bat), ADAM10/17 inhibitor GI254023X (GI) or a combination of both (Bat/GI). As a control, cells were incubated with 0.1% of the solvent DMSO. Supernatants of NCI-N87 cells were analyzed by Western blot for N-terminal fragments of hDsg2 (Dsg2 NTF ) and hCdh1 (Cdh1 NTF ) using antibodies recognizing the extracellular domains of hDsg2 and hCdh1. The overlay of hDsg2 (green) and hCdh1 (red) is presented to exclude possible antibody cross reactions. C 50 ng rCdh1 were incubated with 100 ng recombinant human MMP-7 for 16 h at 37 °C. As indicated, increasing concentrations of the broad range MMP inhibitor Batimastat (Bat) were added. Proteins were separated by SDS-PAGE and analyzed by Western blot. Full length rCdh1 (rCdh1 FL ) and cleavage fragments were detected using an antibody recognizing the extracellular domain of hCdh1. D 50 ng rCdh1 was incubated with 250 ng HpHtrA wt, inactive mutant (SA) or 100 ng active MMP-7 for 16 h at 37 °C. Where indicated broad range MMP inhibitor Batimastat (Bat), ADAM10 inhibitor GI254023X (GI) or a combination of both (Bat/GI) was added. Proteins were separated by SDS-PAGE and analyzed by Western blot. Full length rCdh1 (rCdh1 FL ) and cleavage fragments were detected using an antibody recognizing the extracellular domain of hCdh1. HpHtrA was detected with a polyclonal HpHtrA antibody
Chlorpropamide, supplied by Enamine Ltd, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/t+bruker+biospec+94+20/Chlorpropamide/custom%40en300-7388134%4038346601
Average 94 stars, based on 1 article reviews
chlorpropamide - by Bioz Stars, 2026-09
94/100 stars
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m w protein standard  (Danaher Inc)
93
Danaher Inc m w protein standard
MMP and ADAM10 inhibitors do not block HpHtrA activity. A NCI-N87 cells were infected with H. pylori strains P12 wt, N6 wt and N6 ΔhtrA for 24 h. Where indicated, cells were treated with the broad range MMP inhibitor <t>Batimastat</t> (Bat) and ADAM10 inhibitor GI254023X (GI). Supernatants of infected cells were analyzed by gelatin zymography (upper panel) for proteolytic activity of activated MMPs and by casein zymography (lower panel) for proteolytic activity of secreted HpHtrA from H. pylori P12 wt and N6 wt. As a control, 10 µg of bacterial lysates of H. pylori P12 wt, N6 wt and N6 ΔhtrA were loaded on the zymograms. B NCI-N87 cells were stimulated for 24 h with 50 ng/ml TNFα to induce MMP activation. Where indicated, cells were additionally treated with broad range MMP inhibitor Batimastat (Bat), ADAM10/17 inhibitor GI254023X (GI) or a combination of both (Bat/GI). As a control, cells were incubated with 0.1% of the solvent DMSO. Supernatants of NCI-N87 cells were analyzed by Western blot for N-terminal fragments of hDsg2 (Dsg2 NTF ) and hCdh1 (Cdh1 NTF ) using antibodies recognizing the extracellular domains of hDsg2 and hCdh1. The overlay of hDsg2 (green) and hCdh1 (red) is presented to exclude possible antibody cross reactions. C 50 ng rCdh1 were incubated with 100 ng recombinant human MMP-7 for 16 h at 37 °C. As indicated, increasing concentrations of the broad range MMP inhibitor Batimastat (Bat) were added. Proteins were separated by SDS-PAGE and analyzed by Western blot. Full length rCdh1 (rCdh1 FL ) and cleavage fragments were detected using an antibody recognizing the extracellular domain of hCdh1. D 50 ng rCdh1 was incubated with 250 ng HpHtrA wt, inactive mutant (SA) or 100 ng active MMP-7 for 16 h at 37 °C. Where indicated broad range MMP inhibitor Batimastat (Bat), ADAM10 inhibitor GI254023X (GI) or a combination of both (Bat/GI) was added. Proteins were separated by SDS-PAGE and analyzed by Western blot. Full length rCdh1 (rCdh1 FL ) and cleavage fragments were detected using an antibody recognizing the extracellular domain of hCdh1. HpHtrA was detected with a polyclonal HpHtrA antibody
M W Protein Standard, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/t+bruker+biospec+94+20/Standard+14/10__1007_slash_s11746___004___960___1-74-2-8
Average 93 stars, based on 1 article reviews
m w protein standard - by Bioz Stars, 2026-09
93/100 stars
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t system  (Bruker Corporation)
98
Bruker Corporation t system
MMP and ADAM10 inhibitors do not block HpHtrA activity. A NCI-N87 cells were infected with H. pylori strains P12 wt, N6 wt and N6 ΔhtrA for 24 h. Where indicated, cells were treated with the broad range MMP inhibitor <t>Batimastat</t> (Bat) and ADAM10 inhibitor GI254023X (GI). Supernatants of infected cells were analyzed by gelatin zymography (upper panel) for proteolytic activity of activated MMPs and by casein zymography (lower panel) for proteolytic activity of secreted HpHtrA from H. pylori P12 wt and N6 wt. As a control, 10 µg of bacterial lysates of H. pylori P12 wt, N6 wt and N6 ΔhtrA were loaded on the zymograms. B NCI-N87 cells were stimulated for 24 h with 50 ng/ml TNFα to induce MMP activation. Where indicated, cells were additionally treated with broad range MMP inhibitor Batimastat (Bat), ADAM10/17 inhibitor GI254023X (GI) or a combination of both (Bat/GI). As a control, cells were incubated with 0.1% of the solvent DMSO. Supernatants of NCI-N87 cells were analyzed by Western blot for N-terminal fragments of hDsg2 (Dsg2 NTF ) and hCdh1 (Cdh1 NTF ) using antibodies recognizing the extracellular domains of hDsg2 and hCdh1. The overlay of hDsg2 (green) and hCdh1 (red) is presented to exclude possible antibody cross reactions. C 50 ng rCdh1 were incubated with 100 ng recombinant human MMP-7 for 16 h at 37 °C. As indicated, increasing concentrations of the broad range MMP inhibitor Batimastat (Bat) were added. Proteins were separated by SDS-PAGE and analyzed by Western blot. Full length rCdh1 (rCdh1 FL ) and cleavage fragments were detected using an antibody recognizing the extracellular domain of hCdh1. D 50 ng rCdh1 was incubated with 250 ng HpHtrA wt, inactive mutant (SA) or 100 ng active MMP-7 for 16 h at 37 °C. Where indicated broad range MMP inhibitor Batimastat (Bat), ADAM10 inhibitor GI254023X (GI) or a combination of both (Bat/GI) was added. Proteins were separated by SDS-PAGE and analyzed by Western blot. Full length rCdh1 (rCdh1 FL ) and cleavage fragments were detected using an antibody recognizing the extracellular domain of hCdh1. HpHtrA was detected with a polyclonal HpHtrA antibody
T System, supplied by Bruker Corporation, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/t+bruker+biospec+94+20/BioSpec/pmc08474688-75-7-9
Average 98 stars, based on 1 article reviews
t system - by Bioz Stars, 2026-09
98/100 stars
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phusion polymerase  (Promega)
90
Promega phusion polymerase
MMP and ADAM10 inhibitors do not block HpHtrA activity. A NCI-N87 cells were infected with H. pylori strains P12 wt, N6 wt and N6 ΔhtrA for 24 h. Where indicated, cells were treated with the broad range MMP inhibitor <t>Batimastat</t> (Bat) and ADAM10 inhibitor GI254023X (GI). Supernatants of infected cells were analyzed by gelatin zymography (upper panel) for proteolytic activity of activated MMPs and by casein zymography (lower panel) for proteolytic activity of secreted HpHtrA from H. pylori P12 wt and N6 wt. As a control, 10 µg of bacterial lysates of H. pylori P12 wt, N6 wt and N6 ΔhtrA were loaded on the zymograms. B NCI-N87 cells were stimulated for 24 h with 50 ng/ml TNFα to induce MMP activation. Where indicated, cells were additionally treated with broad range MMP inhibitor Batimastat (Bat), ADAM10/17 inhibitor GI254023X (GI) or a combination of both (Bat/GI). As a control, cells were incubated with 0.1% of the solvent DMSO. Supernatants of NCI-N87 cells were analyzed by Western blot for N-terminal fragments of hDsg2 (Dsg2 NTF ) and hCdh1 (Cdh1 NTF ) using antibodies recognizing the extracellular domains of hDsg2 and hCdh1. The overlay of hDsg2 (green) and hCdh1 (red) is presented to exclude possible antibody cross reactions. C 50 ng rCdh1 were incubated with 100 ng recombinant human MMP-7 for 16 h at 37 °C. As indicated, increasing concentrations of the broad range MMP inhibitor Batimastat (Bat) were added. Proteins were separated by SDS-PAGE and analyzed by Western blot. Full length rCdh1 (rCdh1 FL ) and cleavage fragments were detected using an antibody recognizing the extracellular domain of hCdh1. D 50 ng rCdh1 was incubated with 250 ng HpHtrA wt, inactive mutant (SA) or 100 ng active MMP-7 for 16 h at 37 °C. Where indicated broad range MMP inhibitor Batimastat (Bat), ADAM10 inhibitor GI254023X (GI) or a combination of both (Bat/GI) was added. Proteins were separated by SDS-PAGE and analyzed by Western blot. Full length rCdh1 (rCdh1 FL ) and cleavage fragments were detected using an antibody recognizing the extracellular domain of hCdh1. HpHtrA was detected with a polyclonal HpHtrA antibody
Phusion Polymerase, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/t+bruker+biospec+94+20/pfu+dna+polymerase/pmc03654794-261-6-8
Average 90 stars, based on 1 article reviews
phusion polymerase - by Bioz Stars, 2026-09
90/100 stars
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c57 bks db/db male mice  (GemPharmatech Co Ltd)
90
GemPharmatech Co Ltd c57 bks db/db male mice
MMP and ADAM10 inhibitors do not block HpHtrA activity. A NCI-N87 cells were infected with H. pylori strains P12 wt, N6 wt and N6 ΔhtrA for 24 h. Where indicated, cells were treated with the broad range MMP inhibitor <t>Batimastat</t> (Bat) and ADAM10 inhibitor GI254023X (GI). Supernatants of infected cells were analyzed by gelatin zymography (upper panel) for proteolytic activity of activated MMPs and by casein zymography (lower panel) for proteolytic activity of secreted HpHtrA from H. pylori P12 wt and N6 wt. As a control, 10 µg of bacterial lysates of H. pylori P12 wt, N6 wt and N6 ΔhtrA were loaded on the zymograms. B NCI-N87 cells were stimulated for 24 h with 50 ng/ml TNFα to induce MMP activation. Where indicated, cells were additionally treated with broad range MMP inhibitor Batimastat (Bat), ADAM10/17 inhibitor GI254023X (GI) or a combination of both (Bat/GI). As a control, cells were incubated with 0.1% of the solvent DMSO. Supernatants of NCI-N87 cells were analyzed by Western blot for N-terminal fragments of hDsg2 (Dsg2 NTF ) and hCdh1 (Cdh1 NTF ) using antibodies recognizing the extracellular domains of hDsg2 and hCdh1. The overlay of hDsg2 (green) and hCdh1 (red) is presented to exclude possible antibody cross reactions. C 50 ng rCdh1 were incubated with 100 ng recombinant human MMP-7 for 16 h at 37 °C. As indicated, increasing concentrations of the broad range MMP inhibitor Batimastat (Bat) were added. Proteins were separated by SDS-PAGE and analyzed by Western blot. Full length rCdh1 (rCdh1 FL ) and cleavage fragments were detected using an antibody recognizing the extracellular domain of hCdh1. D 50 ng rCdh1 was incubated with 250 ng HpHtrA wt, inactive mutant (SA) or 100 ng active MMP-7 for 16 h at 37 °C. Where indicated broad range MMP inhibitor Batimastat (Bat), ADAM10 inhibitor GI254023X (GI) or a combination of both (Bat/GI) was added. Proteins were separated by SDS-PAGE and analyzed by Western blot. Full length rCdh1 (rCdh1 FL ) and cleavage fragments were detected using an antibody recognizing the extracellular domain of hCdh1. HpHtrA was detected with a polyclonal HpHtrA antibody
C57 Bks Db/Db Male Mice, supplied by GemPharmatech Co Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/t+bruker+biospec+94+20/c57bl+6j+mice/pm38015723-188-1-28
Average 90 stars, based on 1 article reviews
c57 bks db/db male mice - by Bioz Stars, 2026-09
90/100 stars
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5-bromo-2-chlorobenzoic acid (e-merck 8.14989.0101, 20 g; 84.94 mmol; 1 eq.)  (Merck KGaA)
90
Merck KGaA 5-bromo-2-chlorobenzoic acid (e-merck 8.14989.0101, 20 g; 84.94 mmol; 1 eq.)
MMP and ADAM10 inhibitors do not block HpHtrA activity. A NCI-N87 cells were infected with H. pylori strains P12 wt, N6 wt and N6 ΔhtrA for 24 h. Where indicated, cells were treated with the broad range MMP inhibitor <t>Batimastat</t> (Bat) and ADAM10 inhibitor GI254023X (GI). Supernatants of infected cells were analyzed by gelatin zymography (upper panel) for proteolytic activity of activated MMPs and by casein zymography (lower panel) for proteolytic activity of secreted HpHtrA from H. pylori P12 wt and N6 wt. As a control, 10 µg of bacterial lysates of H. pylori P12 wt, N6 wt and N6 ΔhtrA were loaded on the zymograms. B NCI-N87 cells were stimulated for 24 h with 50 ng/ml TNFα to induce MMP activation. Where indicated, cells were additionally treated with broad range MMP inhibitor Batimastat (Bat), ADAM10/17 inhibitor GI254023X (GI) or a combination of both (Bat/GI). As a control, cells were incubated with 0.1% of the solvent DMSO. Supernatants of NCI-N87 cells were analyzed by Western blot for N-terminal fragments of hDsg2 (Dsg2 NTF ) and hCdh1 (Cdh1 NTF ) using antibodies recognizing the extracellular domains of hDsg2 and hCdh1. The overlay of hDsg2 (green) and hCdh1 (red) is presented to exclude possible antibody cross reactions. C 50 ng rCdh1 were incubated with 100 ng recombinant human MMP-7 for 16 h at 37 °C. As indicated, increasing concentrations of the broad range MMP inhibitor Batimastat (Bat) were added. Proteins were separated by SDS-PAGE and analyzed by Western blot. Full length rCdh1 (rCdh1 FL ) and cleavage fragments were detected using an antibody recognizing the extracellular domain of hCdh1. D 50 ng rCdh1 was incubated with 250 ng HpHtrA wt, inactive mutant (SA) or 100 ng active MMP-7 for 16 h at 37 °C. Where indicated broad range MMP inhibitor Batimastat (Bat), ADAM10 inhibitor GI254023X (GI) or a combination of both (Bat/GI) was added. Proteins were separated by SDS-PAGE and analyzed by Western blot. Full length rCdh1 (rCdh1 FL ) and cleavage fragments were detected using an antibody recognizing the extracellular domain of hCdh1. HpHtrA was detected with a polyclonal HpHtrA antibody
5 Bromo 2 Chlorobenzoic Acid (E Merck 8.14989.0101, 20 G; 84.94 Mmol; 1 Eq.), supplied by Merck KGaA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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MMP and ADAM10 inhibitors do not block HpHtrA activity. A NCI-N87 cells were infected with H. pylori strains P12 wt, N6 wt and N6 ΔhtrA for 24 h. Where indicated, cells were treated with the broad range MMP inhibitor Batimastat (Bat) and ADAM10 inhibitor GI254023X (GI). Supernatants of infected cells were analyzed by gelatin zymography (upper panel) for proteolytic activity of activated MMPs and by casein zymography (lower panel) for proteolytic activity of secreted HpHtrA from H. pylori P12 wt and N6 wt. As a control, 10 µg of bacterial lysates of H. pylori P12 wt, N6 wt and N6 ΔhtrA were loaded on the zymograms. B NCI-N87 cells were stimulated for 24 h with 50 ng/ml TNFα to induce MMP activation. Where indicated, cells were additionally treated with broad range MMP inhibitor Batimastat (Bat), ADAM10/17 inhibitor GI254023X (GI) or a combination of both (Bat/GI). As a control, cells were incubated with 0.1% of the solvent DMSO. Supernatants of NCI-N87 cells were analyzed by Western blot for N-terminal fragments of hDsg2 (Dsg2 NTF ) and hCdh1 (Cdh1 NTF ) using antibodies recognizing the extracellular domains of hDsg2 and hCdh1. The overlay of hDsg2 (green) and hCdh1 (red) is presented to exclude possible antibody cross reactions. C 50 ng rCdh1 were incubated with 100 ng recombinant human MMP-7 for 16 h at 37 °C. As indicated, increasing concentrations of the broad range MMP inhibitor Batimastat (Bat) were added. Proteins were separated by SDS-PAGE and analyzed by Western blot. Full length rCdh1 (rCdh1 FL ) and cleavage fragments were detected using an antibody recognizing the extracellular domain of hCdh1. D 50 ng rCdh1 was incubated with 250 ng HpHtrA wt, inactive mutant (SA) or 100 ng active MMP-7 for 16 h at 37 °C. Where indicated broad range MMP inhibitor Batimastat (Bat), ADAM10 inhibitor GI254023X (GI) or a combination of both (Bat/GI) was added. Proteins were separated by SDS-PAGE and analyzed by Western blot. Full length rCdh1 (rCdh1 FL ) and cleavage fragments were detected using an antibody recognizing the extracellular domain of hCdh1. HpHtrA was detected with a polyclonal HpHtrA antibody

Journal: Cell Communication and Signaling : CCS

Article Title: Identification of Desmoglein-2 as a novel target of Helicobacter pylori HtrA in epithelial cells

doi: 10.1186/s12964-021-00788-x

Figure Lengend Snippet: MMP and ADAM10 inhibitors do not block HpHtrA activity. A NCI-N87 cells were infected with H. pylori strains P12 wt, N6 wt and N6 ΔhtrA for 24 h. Where indicated, cells were treated with the broad range MMP inhibitor Batimastat (Bat) and ADAM10 inhibitor GI254023X (GI). Supernatants of infected cells were analyzed by gelatin zymography (upper panel) for proteolytic activity of activated MMPs and by casein zymography (lower panel) for proteolytic activity of secreted HpHtrA from H. pylori P12 wt and N6 wt. As a control, 10 µg of bacterial lysates of H. pylori P12 wt, N6 wt and N6 ΔhtrA were loaded on the zymograms. B NCI-N87 cells were stimulated for 24 h with 50 ng/ml TNFα to induce MMP activation. Where indicated, cells were additionally treated with broad range MMP inhibitor Batimastat (Bat), ADAM10/17 inhibitor GI254023X (GI) or a combination of both (Bat/GI). As a control, cells were incubated with 0.1% of the solvent DMSO. Supernatants of NCI-N87 cells were analyzed by Western blot for N-terminal fragments of hDsg2 (Dsg2 NTF ) and hCdh1 (Cdh1 NTF ) using antibodies recognizing the extracellular domains of hDsg2 and hCdh1. The overlay of hDsg2 (green) and hCdh1 (red) is presented to exclude possible antibody cross reactions. C 50 ng rCdh1 were incubated with 100 ng recombinant human MMP-7 for 16 h at 37 °C. As indicated, increasing concentrations of the broad range MMP inhibitor Batimastat (Bat) were added. Proteins were separated by SDS-PAGE and analyzed by Western blot. Full length rCdh1 (rCdh1 FL ) and cleavage fragments were detected using an antibody recognizing the extracellular domain of hCdh1. D 50 ng rCdh1 was incubated with 250 ng HpHtrA wt, inactive mutant (SA) or 100 ng active MMP-7 for 16 h at 37 °C. Where indicated broad range MMP inhibitor Batimastat (Bat), ADAM10 inhibitor GI254023X (GI) or a combination of both (Bat/GI) was added. Proteins were separated by SDS-PAGE and analyzed by Western blot. Full length rCdh1 (rCdh1 FL ) and cleavage fragments were detected using an antibody recognizing the extracellular domain of hCdh1. HpHtrA was detected with a polyclonal HpHtrA antibody

Article Snippet: Cells were starved 60 min prior to infection or treated with 10 μM GI254023X (CAS 260264–93-5, blocks ADAM10 and ADAM17, IC 50 5.3 nM and 541 nM, respectively; Sigma-Aldrich, Vienna Austria) and 20 μM Batimastat (CAS 130370-60-4, blocks metalloproteinases including MMP-1, MMP-2, MMP-3, MMP-7, MMP-9, ΔMT1, ADAM8, and ADAM17/TACE, IC 50 = 3, 4, 20, 6, 4, 2.08, 51.3, and 19 nM, respectively, Merck, Darmstadt, Germany).

Techniques: Blocking Assay, Activity Assay, Infection, Zymography, Control, Activation Assay, Incubation, Solvent, Western Blot, Recombinant, SDS Page, Mutagenesis

HpHtrA cleaves Desmoglein-2 on the cell surface of gastric epithelial cells independently of MMPs and ADAM10. NCI-N87 and MKN28 cells were infected with H. pylori P12 wt, N6 wt and N6 ΔhtrA for 24 h or left uninfected (mock). Where indicated, cells were treated with broad range MMP inhibitor Batimastat and ADAM10 inhibitor GI254023X (Bat/GI). A Whole cell lysates of infected NCI-N87 (left panel) and MKN28 cells (right panel) were analyzed by Western blot for full length hDsg2 (hDsg2 FL ). CagA, HpHtrA, and GAPDH were detected as loading controls. B Full length hDsg2 (hDsg2 FL ) of infected NCI-N87 cells (left panel) and infected MKN28 cells (right panel) was quantified and normalized to respective GAPDH loading controls. Data represent mean values ± S.D (n = 4). Asterisks indicate statistically significant differences (*p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001; ns, not significant)

Journal: Cell Communication and Signaling : CCS

Article Title: Identification of Desmoglein-2 as a novel target of Helicobacter pylori HtrA in epithelial cells

doi: 10.1186/s12964-021-00788-x

Figure Lengend Snippet: HpHtrA cleaves Desmoglein-2 on the cell surface of gastric epithelial cells independently of MMPs and ADAM10. NCI-N87 and MKN28 cells were infected with H. pylori P12 wt, N6 wt and N6 ΔhtrA for 24 h or left uninfected (mock). Where indicated, cells were treated with broad range MMP inhibitor Batimastat and ADAM10 inhibitor GI254023X (Bat/GI). A Whole cell lysates of infected NCI-N87 (left panel) and MKN28 cells (right panel) were analyzed by Western blot for full length hDsg2 (hDsg2 FL ). CagA, HpHtrA, and GAPDH were detected as loading controls. B Full length hDsg2 (hDsg2 FL ) of infected NCI-N87 cells (left panel) and infected MKN28 cells (right panel) was quantified and normalized to respective GAPDH loading controls. Data represent mean values ± S.D (n = 4). Asterisks indicate statistically significant differences (*p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001; ns, not significant)

Article Snippet: Cells were starved 60 min prior to infection or treated with 10 μM GI254023X (CAS 260264–93-5, blocks ADAM10 and ADAM17, IC 50 5.3 nM and 541 nM, respectively; Sigma-Aldrich, Vienna Austria) and 20 μM Batimastat (CAS 130370-60-4, blocks metalloproteinases including MMP-1, MMP-2, MMP-3, MMP-7, MMP-9, ΔMT1, ADAM8, and ADAM17/TACE, IC 50 = 3, 4, 20, 6, 4, 2.08, 51.3, and 19 nM, respectively, Merck, Darmstadt, Germany).

Techniques: Infection, Western Blot